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ih inos rabbit novus biologicals nb300 605  (Novus Biologicals)


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    Novus Biologicals ih inos rabbit novus biologicals nb300 605
    Ih Inos Rabbit Novus Biologicals Nb300 605, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fig. 2 DCA induces the expres- sion of nitrosative/oxidative stress markers in pancreatic adenocarcinoma cells. A–C Capan-2 and BxPC-3 cells were treated with DCA (0.7 µM) for 48 h. A <t>iNOS</t> protein expres- sion was analyzed by Western blotting (n = 4, upper panels: representative western blots, lower panels: densitometric analysis of Western blots from independent experiments). B Nitrotyrosine and <t>C</t> <t>4-HNE</t> levels were determined by Western blotting. Similar results were obtained in three independent experiments. Data are plotted as means ± SEM. * indicates p < 0.05, DMSO vs. DCA treated groups. DCA deoxycholic acid; DMSO dime- thyl sulfoxide; iNOS inducible nitric oxide synthase; 4-HNE 4-hydroxynonenal.
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    Fig. 2 DCA induces the expres- sion of nitrosative/oxidative stress markers in pancreatic adenocarcinoma cells. A–C Capan-2 and BxPC-3 cells were treated with DCA (0.7 µM) for 48 h. A <t>iNOS</t> protein expres- sion was analyzed by Western blotting (n = 4, upper panels: representative western blots, lower panels: densitometric analysis of Western blots from independent experiments). B Nitrotyrosine and <t>C</t> <t>4-HNE</t> levels were determined by Western blotting. Similar results were obtained in three independent experiments. Data are plotted as means ± SEM. * indicates p < 0.05, DMSO vs. DCA treated groups. DCA deoxycholic acid; DMSO dime- thyl sulfoxide; iNOS inducible nitric oxide synthase; 4-HNE 4-hydroxynonenal.
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    Fig. 2 DCA induces the expres- sion of nitrosative/oxidative stress markers in pancreatic adenocarcinoma cells. A–C Capan-2 and BxPC-3 cells were treated with DCA (0.7 µM) for 48 h. A iNOS protein expres- sion was analyzed by Western blotting (n = 4, upper panels: representative western blots, lower panels: densitometric analysis of Western blots from independent experiments). B Nitrotyrosine and C 4-HNE levels were determined by Western blotting. Similar results were obtained in three independent experiments. Data are plotted as means ± SEM. * indicates p < 0.05, DMSO vs. DCA treated groups. DCA deoxycholic acid; DMSO dime- thyl sulfoxide; iNOS inducible nitric oxide synthase; 4-HNE 4-hydroxynonenal.

    Journal: Molecular biology reports

    Article Title: The pro- and antineoplastic effects of deoxycholic acid in pancreatic adenocarcinoma cell models.

    doi: 10.1007/s11033-023-08453-x

    Figure Lengend Snippet: Fig. 2 DCA induces the expres- sion of nitrosative/oxidative stress markers in pancreatic adenocarcinoma cells. A–C Capan-2 and BxPC-3 cells were treated with DCA (0.7 µM) for 48 h. A iNOS protein expres- sion was analyzed by Western blotting (n = 4, upper panels: representative western blots, lower panels: densitometric analysis of Western blots from independent experiments). B Nitrotyrosine and C 4-HNE levels were determined by Western blotting. Similar results were obtained in three independent experiments. Data are plotted as means ± SEM. * indicates p < 0.05, DMSO vs. DCA treated groups. DCA deoxycholic acid; DMSO dime- thyl sulfoxide; iNOS inducible nitric oxide synthase; 4-HNE 4-hydroxynonenal.

    Article Snippet: Table 2 Antibodies used in Western blot analyses Antibody symbol Vendor Dilution β-CATENIN Cell Signaling Technology (8480) 1:1000 SLUG Cell Signaling Technology (9585) 1:1000 SNAIL Cell Signaling Technology (3879) 1:1000 CLAUDIN-1 Cell Signaling Technology (13,255) 1:1000 ZO-1 Cell Signaling Technology (8193) 1:1000 E-CADHERIN Cell Signaling Technology (3195) 1:1000 iNOS Novus (NB300-605) 1:1000 4-HNE Abcam (ab46545) 1:1000 ALDH1 Abcam (ab227948) 1:1000 NITROTYROSINE Thermo Fisher (A21285) 1:1000 ACTIN Sigma-Aldrich (A3854) 1:20,000 1 3 1 3 Oligomycin sensitive OCR (baseline—oligomycin) refers to ATP production-linked respiration.

    Techniques: Western Blot